e-Myco™ plus Mycoplasma PCR Detection Kit greatly simplify testing and detection of Mycoplasma contamination in cell cultures. With PCR testing, reliable results are obtained within a few hours, since the presence of contaminant Mycoplasmas can be easily and sensitively detected by simply verifying the bands of amplified DNA fragments after gel electrophoresis. The e-Myco™ plus Mycoplasma PCR Detection has been shown to be a highly sensitive, specific and rapid method for the detection of Mycoplasmas contamination in cell cultures.
An internal control of this product was constructed to identify false negative results in each reaction. The internal control was designed in such a way that the primers set was used to amplify the internal control and target DNA, which were differentiated by size. Furthermore, the sample control was provided with this kit for using in verifying the effectiveness of template DNA. So, You may easily check your sample preparation. In addition, the use of 8-methoxypsoralen (8-MOP) was adopted in this kit. 8-MOP is known to intercalate into double-stranded nucleic acids and form a covalent inter-strand crosslink after photo-activation by incident light at wavelength 320-400 nm, so it is helpful to prevent cross-contamination by PCR products from earlier experiments
■ Premix Type
- This e-Myco™ plus Mycoplasma PCR Detection Kit contains all the components for the PCR reaction. You just add a template and DW.
■ Wide Range of Detectable Mycoplasmas
- You can detect not only five common cell culture-infecting species of mycoplasma but also other various species of Mycoplasma over 8 genus 209
■ Internal Control
- Internal control embedded in the product prevents misjudgment that possibly arises from an erroneous PCR test.
■ Sample Control
- You can verify easily the effectiveness of template gDNA by checking the amplification from sample control.
■ Species Determination
- You can determine the species of Mycoplasma by sequencing the amplified PCR products.
■ Elimination of Carryover Contamination System
- 8-MOP prevents cross-contamination by PCR products.
■ PCR Reaction volume
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20 μl reaction |
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e-MycoTM Mycoplasma PCR Detection Kit
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DNA Polymerase
|
2.5U
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Chemical Stabilizer
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1X
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Loading Buffer
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1X
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dNTPs
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250mM each
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Tris-HCl (pH 8.3)
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10mM
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| KCl |
50mM
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| MgCl2 |
1.5mM
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Mycoplasma Primer Sets
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10pmol/each
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| Internal Control |
| 8-MOP (dissolved in DMSO) |
|
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e-Myco™ Mycoplasma PCR Detection Kit
|
100㎕(1U/㎕)
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| DNase/RNase-free Distilled Water |
2 ml
|
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Control DNA
(genomic DNA from M. fermentans-infected K562)
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Overview of Mycoplasma Detection
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