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| Mycoplasma Detection Kit 1 » M-Solution™ 1-2 Antibiotics (for Mycoplasma) |
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| M-Solution™ 1-2 Antibiotics (for Mycoplasma) |
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Mycoplasma are common and serious contaminants of cell cultures. It has been shown that 30-87% of cell cultures are infected with mycoplasma. Many mycoplasma contaminations, particularly in continuous cell lines, grow slowly and do not destroy host cells but are still able to affect various parameters, leading to unreliable or false results. These effects include changes in metabolism, growth, viability, DNA, RNA, protein synthesis, and morphology etc. Testing for mycoplasma infection is essential quality control tool to assure accurate research and reliable biotechnological products.
M-SolutionTM 1-2 Antibiotics (100x) are a combination of antibiotics, which have been shown to be effective in the elimination of mycoplasma species that account for 90% of the contamination found in cell cultures. M-Solution¢â 1-2 Antibiotics (100x) are antibiotic formulations designed to treat Mycoplasma infected cell cultures. M-Solution¢â 1 Antibiotic is a solution based on the antibiotic Tiamutin. M-Solution¢â 2 Antibiotic is a solution based on the antibiotic Minocycline, which is a member of the Tetracycline group. These two antibiotics have been shown to be effective in eliminating the mycoplasma species frequently present in contaminated cell cultures. Furthermore, the mycoplasmas do not develop resistance to these antibiotics – a common occurrence with other antibiotic treatment methods. The two antibiotic solutions are generally used sequentially, in combination. The two antibiotic solutions are used sequentially over a period of 1 week, and the cycle is repeated 2-3 times as required. The treatment is quite easy to perform and when used according to the following protocol, no cytotoxic effects will occur.
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- Highly Effiency
- No cytotoxic effect
- Easy-to-use solutions |
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- M-SolutionTM 1-2 Antibiotics (100x) is a sterile, ready-to-use solution. Store the original container at -20C.
- Always avoid multiple freeze-thaw cycles or exposure to frequent temperature changes.
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- M-SolutionTM 1 Antibiotic (100x)
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10§¢
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- M-SolutionTM 2 Antibiotic (100x)
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10§¢
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 Estimate the level of elimination of mycoplasma |
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Fig. 1. Result for the various cell number
To estimate the level of elimination of mycoplasma for the various cell number, it was performed with e-MycoTMMycoplasma PCR Detection Kit (iNtRON, Cat. No. 25233).
Lane M: 100bp Ladder DNA Marker (Cat. No. 24022),
Lane 0 : Negative control, Lane 1 : 2x105 cells, Lane 2 : 1x105 cells, Lane 3 : 5x104 cells,
Lane 4 : 2.5x104 cells, Lane 5 : 1.25x104 cells, Lane 6 : 6.25x103 cells,
Lane 7 : 3.125x103 cells, Lane 8 : 1.56x103 cells, Lane 9 : 7.8x102 cells,
Lane 10 : 3.9x102 cells, Lane 11 : 1.9x102 cells, Lane 12 : 98 cells,
Lane 13 : 49 cells, Lane 14 : 24 cells, Lane 15 : 12 cells
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Fig. 2. Result for the various concentration of template DNA
To estimate the level of elimination of mycoplasma for the various concentration of template DNA, it was performed with e-MycoTM Mycoplasma PCR Detection Kit (iNtRON, Cat. No. 25233).
Lane M : 100bp Ladder DNA Marker (Cat. No. 24022),
Lane 0 : Negative control, Lane 1 : 100ng, Lane 2 : 50ng, Lane 3 : 25ng,
Lane 4 : 12.5ng, Lane 5 : 6.3ng, Lane 6 : 3.2ng, Lane 7 : 1.6ng,
Lane 8 : 800pg, Lane 9 : 400pg, Lane 10 : 200pg, Lane 11 : 100pg,
Lane 12 : 50pg, Lane 13 : 25pg, Lane 14 : 12.5pg, Lane 15 : 6.3pg |
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