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| Nucleic Acid 1 » Maxime™ PCR PreMix Kit (i-Taq), 20 ul (96 Tubes) |
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| Maxime™ PCR PreMix Kit (i-Taq), 20 ul (96 Tubes) |
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Description
Maxime PCR PreMix Kit is mixed every component. Taq DNA Polymerase, dNTP mixture,
and reaction buffer, etc. in one tube can be used for each PCR reaction. It also contains Loading dye. It is required just to add template and primer for PCR reaction.
High Purity Enzyme
DNA Polymerase, using the anion exchange chromatography, so we can attain 95~99% of pure polymerase.
Convenience
Because Maxime PCR PreMix Kit is mixed every component, just add template DNA, primer set and adjust total volume with water.
Reproducibility
We quoted ISO 9001 and 14001, as perform special QC, we guarantee regular quality
Guarantee Date
Store at -20¡ֶC; under this condition, it is stable for least a year.
iNtRON use only pure and refined Taq
We guarantee stable and same result as purified Taq
Comparison with different company kit - 1 
Total RNA was purified from SNU-1 using easy BLUE Total RNA Extraction Kit (Cat. No. 17061). Then, the first strand of cDNA was synthesized using Power cDNA Synthesis Kit(Cat.No. 25011). After diluted the cDNA mixture as indicates, the RT-PCR reaction was performed.
Lane M, 100bp Ladder DNA Marker Lane 1, undiluted cDNA Lane 2, 1/2 diluted cDNA Lane 3, 1/4 diluted cDNA Lane 4, 1/8 diluted cDNA Lane 5, 1/16 diluted cDNA
Comparison of Maxime PCR PreMix (i-StarTaq) and Company A's hot start PreMix system by amplifying 570bp DNA fragment (GAPDH). Total RNA was purified from mouse cells using easy-BLUETM Total RNA Extraction Kit (Cat. No. 17061). And then, the first strand of cDNA was synthesized using Power cDNA Synthesis Kit (Cat. No. 25011). After diluting the cDNA mixture as indicates, the RT-PCR reaction was performed.
A, Company A B, iNtRON¡¯s Maxime PCR PreMix (i-StarTaq)
Lane M, 100bp Ladder DNA Marker Lane 1, undiluted cDNA Lane 2, 1/2 diluted cDNA Lane 3, 1/4 diluted cDNA Lane 4, 1/8 diluted Cdna Lane 5, 1/16 diluted cDNA
Comparison with different company kit - 2
Company A Maxime (i-StarTaq) 
fyuA (780bp), tsh (420bp) multiplex PCR result of using Maxime PCR PreMix (i-StarTaq) and Company B HotStart PCR PreMix with E.coli Genomic DNA template.
Lane M : 100 bp DNA Ladder Lane NC : Negative control Lane 1 : 10 ng gDNA Lane 2 : 2 ng gDNA Lane 3 : 400 pg gDNA
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