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| Nucleic Acid 1 » Maxime™ RT-PCR PreMix Kit, 20 ul (96 Tubes) |
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| Maxime™ RT-PCR PreMix Kit, 20 ul (96 Tubes) |
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- Description
- Normal RT-PCR method is that RT- PCR procedure used by DNA polymerase for
- cDNA synthesis procedure are reacted in each tubes. However, this method is
- very inconvenient and can have cross contamination by sample¡¯s carry over.
- For these problems and inconvenience, Maxime¢ג RT-PCR PreMix Kit made a product
- that is include every container for DNA polymerase and each reaction mixture for you
- and can do first-strand cDNA synthesis and PCR from total RNA or mRNA template
- continually in a tube.
High Purity Enzyme
- DNA Polymerase, using the anion exchange chromatography, so we can attain
- 95~99% of pure polymerase.
Convenience
- The product is mixed every component. RTase, i-StarTaq¢ג DNA Polymerase,
- dNTP mixture, reaction buffer, and so on- in one tube for 1 rxn PCR.
- It contain Loading dye. So, after PCR, we can check it immediately.
Reproducibility
- We quoted ISO 9001 and 14001, as perform special QC, we guarantee regular quality.
Guarantee Date
- Store at -20¡ֶC for one year.
iNtRON¡¯s Advantages
- Add only RNA template for RT.
- Eliminate complicated procedure such as incubation step.
Comparison with different kit
- Company A Maxime¢ג RT-PCR Premix
- Comparison of Maxime RT-PCR PreMix Kit and Company A's RT-PCR PreMix
- system Kit by diagnosis of Newcastle disease (common type).
- 108.0EID50/0.1 ml of allantoic fluid were 10-fold dilution, then total RNA were isolated
- using Viral Gene- spin¢ג Viral DNA/RNA Extraction Kit (Cat.No. 17151).
- From total RNA, the synthesized first strand cDNA and PCR reaction were performed
- using ONE-STEP RT-PCR PreMix Kit and Maxime RT-PCR PreMix Kit.
- Lane M, 100bp Ladder DNA Marker Lane 1, 10-6 dilution
- Lane 2, 10-7 dilution Lane 3, 10-8 dilution
- Maxime¢ג RT-PCR PreMix Company A
- Comparison of Maxime RT-PCR PreMix and Company A's RT-PCR PreMix system
- by amplifying 570bp DNA fragment (GAPDH). Total RNA was purified from SNU-1
- using easy-BLUETM Total RNA Extraction Kit(Cat. No. 17061).
- And then, RT-PCR reaction was performed using Maxime RT-PCR PreMix
- and different company¡¯s RT-PCR PreMix Kit.
- Lane M, 100bp Ladder DNA Marker Lane 1, 2ng total RNA
- Lane 2, 200pg total RNA Lane 3, 20pg total RNA
- Lane 4, 2pg total RNA Lane 5, 200fg total RNA
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