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| Protein Staining and other Solutions 1 » SMART™ Micro BCA Protein Assay Kit (2,000 Test) |
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| SMART™ Micro BCA Protein Assay Kit (2,000 Test) |
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The SMART¢â micro BCA protein assay kit (for Micro Assay) is a detergent-compatible bicinchoninic acid formulation for the colorimetric detection and quantitation of total protein. An adaptation of the SMART¢â BCA Protein Assay Kit (for Standard Assay), SMART¢â micro BCA Protein Assay Kit (for Micro Assay) has been optimized for use with dilute protein samples (2-40 ug/ml).
The purple-colored reaction product of this assay is formed by the chelation of two molecules of BCA with one cuprous ion. This water-soluble complex exhibits a strong absorbance at 562 nm that is nearly linear with increasing protein concentrations.
The macromolecular structure of protein, the number of peptide bonds and the presence of four particular amino acids(cystein, cystine, tryptophan and tyrosine)are reported to be responsible for color formation with BCA. Studies with di-, tri- and tetrapeptides suggest that the extent of color formation caused by more than the mere sum of individual colorproducing functional groups.
SMART¢â micro BCA Protein Assay Kit (for Micro Assay) uses concentrated solutions and a protocol that utilizes an extended incubation time at an elevated temperature (60¡É, Test Tube Procedure only). The result is an extremely sensitive colorimetric protein assay in a microplate or test tube assay format. |
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- Colormetric method; read at 562 nm
- Compatible with most ionic and nonionic detergents
- A very sensitive reagent for dilute protein samples
- Linear working range for BSA: 0.5-20 ug/ml
- Less protein-protein variation that dye-binding methods
- All kit reagents stable at room temperature for 2 years
- Working solution is stable for 24 hours
- Convenient microplate format |
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* Solution A
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100 §¢
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* Solution B
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96 §¢
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* Solution C
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4 §¢
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* BSA solution (128§¶/ml)
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1 §¢ x 10 ea
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Storage at RT
(If either Solution A or Solution B precipitates upon shipping in cold weather or during long-term storage, dissolve precipitates by gently warming and stirring solution. Discard any kit reagent that shows discoloration or evidence of contamination. ) |
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 Improved sensitivity & Linear working range |
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Fig. 1. Comparison of sensitivity of protein assay
SMART¢â BCA Protein Assay Kit (for Micro Assay) shows improved sensitivity higher than competitor product as 15-20%.
The color response was estimated micro well plate reader (37¡É/120 minute incubation) |
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| HIGH FIDELITY |
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Fig. 2. Typical color response curves for BSA, Chymotrypsin and Lysozyme using the Microplate Protocol.
Absorbance ratios (562 nm) for proteins relative to BSA. The SMARTTM BCA Protein Assay Kit (for Micro Assay) showed higher sensitivity (slope) than Competitor. However, The Protien-to-Protein variations showed similar patters.
A; SMART¢â Micro BCA Protein Assay Kit, B; Competitor A Product |
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