Applications

• Electro Elution - fits most horizontal electrophoresis units
• Protein - more than 60% recovery
  • Preparation of protein samples for MALDI-MS
  • Recovery of gel purified proteins for immunization of lab animals
  • Recovery of proteins and simultaneous removal of empholytes from proteins run on a iso-electric focusing gels
  • extraction of protein - protein or protein - nucleic acid complexes from gel
  • extraction of native protein from native gel for biological assays
• Nucleic Acid - Greater than 90% recovery, isolate ANY size effeciently
• Extraction of DNA from agarose or polyacrylomida gels for cloning or sequencing.
• Extraction of oligo's from polyacrylomids gel for PCR, cloning, or primer extension.
• Extraction of RNA from polyacrylomide gel for structure analysis and RNA processing analysis, splicing analysis, bother
• Extraction of protein RNA or protein RNA complexes from agarose or polyacrylomide gel for fast printing analysis.
•  

Protein preparation for MALDI-TOF-MS

Introduction:

Sodium dodecylsulfate-polyacrylamide gel electrophoresis (SDS-PAGE) is a widely used technique for the separation and estimation of the molecular weight of individual proteins

Accuracy of molecular weight determination & characterization is limited by protein low recovery. Recently MALDI-TOF-MS has found widespread use for the determination of molecular mass of intact proteins isolated from gels. Isolation of proteins from gels with the newly developed GeBAflex-tube electro-elution system delivers over 80% recovery yields. Combination of SDS-PAGE, GeBAflex-tube electro-elution system, and MS buffer removing SDS (included in kit), delivers MALDI-TOF-MS,  greater accuracy determination of protein molecular. Now we can analyze low & difficult protein, such as integral membrane proteins (hydrophobic) or high molecular masses protein.

The unique method provides a powerful means for characterizing endogenous proteins of wide molecular weight range separated by SDS-PAGE. The combinations of the three methods provide significantly improve protein yield and SDS free samples. The end result is a MALDI-MS analysis with greater sensitivity. GeBAflex-tube tool provide high protein yield recovery, MS buffer contained in GeBAflex-tube kit clean SDS thoroughly.

PRODUCT CATALOG  REFERENCE

Minimum recovery of protein from polyacrylamide gel: >60%

Maximum volume: 250µl

 Maximum volume: 800µl

GeBAflex-tube Maxi:   Gel slice size: 1x2cm

Maximum Volume: 3000µl

Membrane MWCO: 3500, 6000-8000 and 12000-14000 Da.
Purity: No contamination with Protease, RNase, DNase and PCR product free.
Membrane: ultra-clean, sulfur and heavy metal free and EDTA treated.
Single use unit, no need for cleaning and autoclave. Single step process.        

 High throughput electro elution with GeBAflex-tube using standard electrophorese unit.
Picture show 4 Midi GeBAflex-tube electro eluting, typical 50kDa/ 80m?n.

High throughput electro elution with GeBAflex-tube using standard Maxi electrophoresis unit.

Picture show 24 Midi GeBAflex-tube electro eluting, typical 50kDa/ 80m?n.

A Maxi GeBAflex-tube inside Maxi-supporting tray.  

Mini GeBaflex-tube   Vol.  10-250µl,    Max. gel slice size for electro elution: 0.4x1cm

Midi GeBaflex-tube   Vol.  50-800µl,   Max. gel slice size for electro elution: 0.5x1cm

Maxi GeBaflex-tube  Vol100-3000µl,   Max. gel slice size for electro elution: 1x2cm

GeBAflex-tube Midi:    Gel slice size: 0.5 x 1.1cm

Mini GeBAflex-tube:    Gel slice size: 0.4 x 1.1cm

30 Midi GeBAflex-tube, 3500 cut-off, buffers, supporting tray, floating rack, handbook