Protein preparation for MALDI-TOF-MS
Introduction:
Sodium dodecylsulfate-polyacrylamide gel electrophoresis (SDS-PAGE) is a widely used technique for the separation and estimation of the molecular weight of individual proteins
Accuracy of molecular weight determination & characterization is limited by protein low recovery. Recently MALDI-TOF-MS has found widespread use for the determination of molecular mass of intact proteins isolated from gels. Isolation of proteins from gels with the newly developed GeBAflex-tube electro-elution system delivers over 80% recovery yields. Combination of SDS-PAGE, GeBAflex-tube electro-elution system, and MS buffer removing SDS (included in kit), delivers MALDI-TOF-MS, greater accuracy determination of protein molecular. Now we can analyze low & difficult protein, such as integral membrane proteins (hydrophobic) or high molecular masses protein.
The unique method provides a powerful means for characterizing endogenous proteins of wide molecular weight range separated by SDS-PAGE. The combinations of the three methods provide significantly improve protein yield and SDS free samples. The end result is a MALDI-MS analysis with greater sensitivity. GeBAflex-tube tool provide high protein yield recovery, MS buffer contained in GeBAflex-tube kit clean SDS thoroughly.
PRODUCT CATALOG REFERENCE
Minimum recovery of protein from polyacrylamide gel: >60%
Maximum volume: 250µl
Maximum volume: 800µl
GeBAflex-tube Maxi: Gel slice size: 1x2cm
Maximum Volume: 3000µl
Membrane MWCO: 3500, 6000-8000 and 12000-14000 Da.
Purity: No contamination with Protease, RNase, DNase and PCR product free.
Membrane: ultra-clean, sulfur and heavy metal free and EDTA treated.
Single use unit, no need for cleaning and autoclave. Single step process.
High throughput electro elution with GeBAflex-tube using standard electrophorese unit.
Picture show 4 Midi GeBAflex-tube electro eluting, typical 50kDa/ 80m?n.
High throughput electro elution with GeBAflex-tube using standard Maxi electrophoresis unit.
Picture show 24 Midi GeBAflex-tube electro eluting, typical 50kDa/ 80m?n.
A Maxi GeBAflex-tube inside Maxi-supporting tray.
Mini GeBaflex-tube Vol. 10-250µl, Max. gel slice size for electro elution: 0.4x1cm
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Product
|
Unit
|
MWCO
|
Catalog
|
|
GeBAflex-tube
|
10
|
6000-8000
|
TO70-6-10 |
|
GeBAflex-tube
|
30
|
6000-8000
|
TO70-6-30
|
|
GeBAflex-tube
|
10
|
12000-14000
|
TO70-12-10
|
|
GeBAflex-tube
|
30
|
12000-14000
|
TO70-12-30
|
Midi GeBaflex-tube Vol. 50-800µl, Max. gel slice size for electro elution: 0.5x1cm
|
Product
|
Unit
|
MWCO
|
Catalog
|
|
GeBAflex-tube
|
10
|
3500
|
TO10
|
| |
15
|
3500
|
TO11
|
| |
20
|
3500
|
TO14
|
| |
30
|
3500
|
TO12
|
| |
10
|
6000-8000
|
TO20
|
| |
15
|
6000-8000
|
TO21
|
| |
20
|
6000-8000
|
TO24
|
| |
30
|
6000-8000
|
TO22
|
Maxi GeBaflex-tube Vol100-3000µl, Max. gel slice size for electro elution: 1x2cm
|
Product
|
Unit
|
MWCO
|
Catalog
|
|
GeBAflex-tube
|
5
|
3500
|
TO30
|
|
GeBAflex-tube
|
15
|
3500
|
TO35
|
|
GeBAflex-tube
|
5
|
6000-8000
|
TO40
|
|
GeBAflex-tube
|
15
|
6000-8000
|
TO45
|
|
GeBAflex-tube
|
5
|
12000-14000
|
TO50
|
|
GeBAflex-tube
|
15
|
12000-14000
|
TO55
|
 20 Midi GeBAflex-tube, 6000-8000 cut-off, buffers, supporting tray, floating rack, handbook
GeBAflex-tube Midi: Gel slice size: 0.5 x 1.1cm
Mini GeBAflex-tube: Gel slice size: 0.4 x 1.1cm
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