shRNA/miRNA cloning service
l Cloning service for your shRNA/miRNA
l Your shRNA/miRNA will be cloned under the H1 promoter
l Your clone will be confirmed with sequencing.
l Your vector can be used either a constitutive expressed or Tet inducible way
l Requires 2 weeks.
l Supply 2 ug of vector DNA together with a negative control vector
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* Notes
Supplied DNA vector information
Customer pick target sequence of shRNA and its target gene from human, mouse, or rat.
2.You may pick any length of stem (19 to 30) or sequence of loop for your shRNA
Genolution will cloning the shRNA into an expressed vector.
4.
The expression of shRNA can be constitutively or conditionally expressed using the TetR system.
5.You will have
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DNA vector with shRNA that can be expressed constitutively or conditionally
•Actual sequencing data for your vector
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