II. RNAi validation service
l The only RNAi validation service in the world
l Your siRNA/miRNA/shRNA will be synthesized and transfected into a cell line for an actual RNAi efficacy validation.
l RNAi efficacy will be validated with qPCR including all experimental controls
l Service supply
- siRNA synthesis and transfection
- qPCR primer sequence
- supply 2 nmole validated siRNA and qPCR data
Genolution siRNA Validation report (Example)
date :
Thank you for using Genolution’s RNAi validation service. This is the only service in the world for an actual RNAi validation of your siRNA/shRNA/miRNA.
Description
1. Real Time PCR primer test
Gene Name : HSP27
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Forward Primer :
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5’ CTACATCTCCCGGTGCTTCA 3’
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Reverse Primer :
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5’ GGATGGTGATCTCGTTGGAC 3’
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<Example>
1. Actin : 180
2. HSP27: 149
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(Ct value : 17.43)
We confirm your qPCR product by the peak of qPCR reaction as well as the product size on an agarose gel. If the product doesn’t show the expected size, a new primer set will be designed.
2. < HSP27siRNA validation information>
Gene Information : [X54079.1]Human mRNA for heat shock protein HSP27
Target sequence for validation
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siRNA Name
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Target sequence (5’-3’)
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Target 1
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XXXXXXXXXXXXXXXXXXXXXXXXX
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Target 2
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XXXXXXXXXXXXXXXXXXXXXXXXX
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Target 3
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XXXXXXXXXXXXXXXXXXXXXXXXX
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Target 4
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XXXXXXXXXXXXXXXXXXXXXXXXX
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3. Real time RT PCR data
HSP27 siRNA 10nM in 20% Hela and validation in 24hr
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si RNA
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primer
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ct
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△ct
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△△ct
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2^-△△ct
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Reduction(%)
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Luci
(negative control)
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actin
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18.8
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Vim
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20.75
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1.95
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HSP27
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18.35
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-0.45
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Vim
(positive control)
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actin
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18.96
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Vim
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24.26
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5.3
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