FroggaBio.com

Cat. #	Product Name	Size	Storage temp.
05-080-1B	BIOTARGET™-1 w/o L-Glutamine	100 ml	2-8ºC
05-080-1A	BIOTARGET™-1 w/o L-Glutamine	500 ml	2-8ºC

BIOTARGET™-1 Advancing the field of Immunotherapy

BIOTARGET™-1 serum-free medium developed and optimized specifically for culturing peripheral blood mononuclear cells (lymphocytes and monocytes).

Advantages:

Xeno-free
Chemically defined
No added cytokines
Comprehensive validation studies
CE Mark
Manufactured in cGMP compliant, ISO 13485:2003 and ISO 9001:2008 certified facility
Available in many customized packaging configurations, including Sartorius-Stedim Cultibags

Applications:

Mitogen mediated activation of mononuclear cells (PHA, CON.A, OKT-3)
Lymphoid cell mediated activation of mononuclear cells (RAJI, PEER, BA, MOLT-4, JURKAT)
IL-2 and IL-3 production from mononuclear cells
Long-term post- activation culture of mononuclear cells
LAK / TIL cell generation by mononuclear cell interleukin-2 mediated activation
Natural killer cell (NK) generation by mononuclear cell activation
Cytotoxic T cell generation by mononuclear cell activation
Activation of macrophages
Investigating various cytokines’ influence on the production of mononuclear cell sub-populations
HIV proliferation
Retrovirus proliferation in T cells for vaccine development
Cytokine production in human mixed leukocyte reaction (MLR). BIOTARGET™-1 was found as effective as serum containing medium in supporting leukocyte proliferation and IL-2 secretion.

Introduction Serum Free Medium

Protocol

References:

1. E. Elinav, N. Adam, T. Waks and Z. Eshhar. Amelioration of Colitis by genetically engineered murine regulatory T cells redirected by antigen-specific chimeric receptor. Gastroenterology, Vol. 136, Issue 5, pp. 1721-1731, 2009

2. Yang-Ming Tseng, Sheng-Yi Chen, Chien-Hung Chen, Yi-Ru Jin, Shih-Meng Tsai, Ing-Jun Chen, Jang-Hwa Lee, Chzng-Cheng Chiu and Li-Yu Tsai. Effects of Alcohol-Induced Human Peripheral Blood Mononuclear Cell (PBMC) Pretreated Whey Protein Concentrate (WPC) on Oxidative Damage. Journal of Agricultural and Food Chemistry, 56 (17), pp 8141–8147, 2008

3. Q. Leng, Z. Bentwich and G. Borkow. Increased TGF-ß, Cbl-b and CTLA-4 levels and immunosuppression in association with chronic immune activation. International Immunology 18(5):637-644, 2006

4. D. Melamed, O. Messika, L. Glass-Marmor and A. Miller. Modulation of matrix metalloproteinase-9 (MMP-9) secretion in B lymphopoiesis. International Immunology 18(9):1355-1362, 2006

5. C. Rabinowitz and B. Rinkevich. Epithelial cell cultures from Botryllus schlosseri palleal buds: accomplishments and challenges. Methods in Cell Science, Vol. 25, Numbers 3-4, 2004

6. F. Martí, E. Bertran, M. Llucià, E. Villén, M. Peiró, J. Garcia and F. Rueda. Platelet factor 4 induces human natural killer cells to synthesize and release interleukin-8. Journal of Leukocyte Biology 72:590-597, 2002

7. A. Bishara, R. Malka, C. Brautbar, V. Barak, I. Cohen and E. Kedar.

Cytokine production in human mixed leukocyte reactions performed in serum-free media.

Journal of Immunological Methods, Vol. 215, Issues 1-2, pp.187-190, 1998

8. G. Kampen, L. Poulsen, C. Reimert and P. Skov.

A method for production and determination of histamine releasing activity from human
peripheral blood mononuclear cell. Journal of Immunological Methods, Vol. 210, Issue 2, pp. 185-193, 1997

9. S. Morecki, Y. Gelfand, S. Levi, A. Nagler, R. Condiotti, C. Nabet, A. Ackerstein, S. Slavin. Activated long-term peripheral blood cultures as preparation for adoptive alloreactive cell therapy in cancer patients. Journal of Hematotherapy, 6 (2), pp. 115-124, 1997

10. Malka R.; Brautbar C.; Kedar E.; Cohen I.; Bishara A. Human mixed leukocyte reaction (MLR) performed in serum-free media and serum-containing medium. Human immunology, vol. 44, supp. 1, pp. 137, 1995