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| Serum-Free Media (SFM) 1 » BIOGRO-1 Serum-Free Media Supplement, 2ml |
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| BIOGRO-1 Serum-Free Media Supplement, 2ml |
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| Products |
| Cell Culture > Serum Free Medium Products > BIOGRO-1 SFM Supplement |
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Cat. #
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Product Name
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Size
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Storage temp.
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05-600-1
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BIOGRO-1 Serum-Free Media Supplement
50-Fold Concentrate
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T 2ml
D 10 ml
C 20 ml
B 100 ml
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-20ºC
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In addition to our line of ready-to-use serum-free media products, we have developed BIOGRO-1 and BIOGRO-2 for those customers who prefer to prepare final media on their own.
The BIOGRO-1 formulation contains serum albumin.
The BIOGRO-2 (Cat. no. 05-610-1) formulation is totally free of serum albumin, and the concentration of total protein is therefore less than 18 micrograms per ml in the final media.
BIOGRO-1 & 2 are 50-fold concentrations.
Applications:
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Culture of murine myeloma cells (NS-1, NS-0/1, SP2)
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Culture of mouse-mouse hybridoma cells (from NS-1, NS-0/1, SP2, X63-Aag8-653, BW-S147)
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Culture of rat-mouse, rat-rat, and human-human hybridomas
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Monoclonal antibody production
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Culture of other human, murine and avian lymphocytes
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Viral production (such as HEV virus from Turkey B-lymphoblastiod cell lines)
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Culture of various anchorage-dependent cells (such as human osteosarcoma, human epithelial, and thyroid cells)
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Maintenance of most anchorage-dependent cells during long production periods
Method of use:
The BIOGRO products are 50 fold concentrates and therefore are recommended for use at a concentration of 2% (although in some cases 1% may be sufficient). DMEM: F-12 (1:1) has found to be most generally effective as the basal media, but many cell lines grow well with RPMI, DMEM or Iscove’s. Glutamine and antibiotics should be added to the final formulation.
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