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| Stem Cell Products 1 » AF NutriStemTM hESC XF |
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NutriStemTM .jpg)
Xeno-Free Medium for Human ES & iPS Cell Culture
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Product Name: AF NutriStemTM hESC XF
Catalogue number: 05-102-1B Qty/Pk: 100ml
Catalogue number: 05-102-1A Qty/Pk: 500ml
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Defined, xeno-free, serum-free medium, designed to support the growth of human embryonic stem cells (hESC) and human induced pluripotent stem cells (hiPSC)
Traditional human Embryonic Stem Cells culture methods require the use of mouse or human fibroblast feeder layers, or feeder-conditioned medium. These culture methods are labor-intensive, hard to scale up, and it is difficult to maintain human ES cells undifferentiated due to undefined conditions.
NutriStem™ media were developed by Biological Industries with a leading group in stem cell research, to enable the maintenance and expansion of human ES and iPS cells with feeder cells or on feeder-independent culture.
Human ES cells (i.e., H9.2, I6, I3.2, H1) cultured in NutriStem™ media displayed:
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The ability to self-renew, by expansion in feeder-free culture conditions (Matrigel), on human-feeder layer (HFF) or on Mouse feeder cells (MEFs);
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Expression of hESC specific transcription factors and antigens;
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Pluripotency by the ability to differentiate into the three germ layers (endoderm, ectoderm and mesoderm) in-vitro by EB formation and in-vivo by teratoma formation in SCID mice; and
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Human ES cell normal phenotype and genotype
NutriStem™ human ES & iPS cell culture media provides:
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Consistent media performance and predictable cellular behavior derived from a defined xeno-free formulation
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Increased reproducibility shown in long-term growth of over 50 passages
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Time saving in eliminating the need to prepare feeder cells when choosing to culture on feeder-free layer
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Cost efficiency obtained in low human recombinant growth factor concentrations (rh bFGF, rh TGFβ)
Featurs
- Albumin-Free
- Optimized for feeder-dependent culture (i.e. HFF and MEF)
- Superior performance on HFF feeder layer
- Ready-to-use
- When culturing on feeder-free layer (i.e. Matrigel) an addition of 5% of Bio-Pure Human Serum Albumin (HSA solution, 10%) (Cat.# 05-720-1) is required.
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Storage
Store at -20ºC |
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Stability
18 months
Quality Control
AF NutriStem™ hESC is routinely tested for optimal maintenance and expansion of undifferentiated hESC. Additional standard evalutions are pH, osmolality, endotoxins and sterility tests.
Reference
Human and mouse adipose-derived cells support feeder-independent induction of pluripotent stem cells. Shigeki Sugii, Yasuyuki Kida, Teruhisa Kawamura, Jotaro Suzuki, Rita Vassena, Yun-Qiang Yin, Margaret K. Lutz, W. Travis Berggren, Juan Carlos Izpisúa Belmonte, and Ronald M. Evans. (sent for review September 3, 2009)
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