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Mouse embryonic stem (ES) cells are pluripotent cells derived from the inner cell mass of the blastocyst. Undifferentiated ES cells can be maintained in-vitro for extended periods without loss of their capacity to differentiate to all cell lineages when reimplanted back into a blastocyst. ES cells may differentiate in-vitro to a variety of cell types including neuronal, muscle, endothelial and hematopoietic progenitors. General culture conditions are well established and usually require undifferentiated ES cells to be grown on inactive feeder cell layer or on gelatin-coated plates with Leukemia Inhibitory Factor (LIF) in the culture medium. Gelatin solution (0.1%) is intended for coating cell culture flasks or plates used for the growth of mouse ES cells without feeder layer and with the addition of LIF to the culture medium.
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