RevoScript™ RT PreMix Kit contained in the kitis aproprietary novel avian thermostable single polypeptide RTase derived from an avian retrovirus (Reticuloendotheliosisvirus;REV).REV is a retrovirus that is immunologically,morphologically and structurally distinct from the leucosis/ sarcoma group of avian retroviruses. In addition, REV has a significant phylogenetic relationship with mammalian C-type retroviruses. Because of these characteristics of REV, the RTase derived from REV contains the good qualities of both the avian retrovirus-originated RTases (more stable at higher temperatures) and the mammalian C-type retrovirus-originated RTases (single polypeptide)
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The Novel Recombinant RTase from Avian Retrovirus(75kDa) |
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-Novel Cloned RTase from Avian REV Virus |
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Thermostable RTase (37~60℃, 50℃ optimal) |
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- Ideal for cDNA synthesis for secondary structure RNA with dynamic activation temperature upto 60c. |
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Don't need the Preheating Step |
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-Don't need to the preheating step to unwind the secondary structure of RNA. |
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Ultra Sensitive efficiency of cDNA synthesis |
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- Ultra sensitive efficiency of cDNA synthesis ,even 0.3pg level of RNA . |
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Suitable for the full Length cDNA synthesis. (Above 9Kb) |
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- Design to maximize the synthesis efficiency of large size cDNA synthesis. |
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Ready to Use |
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-Provided by Dried premix containing all components in one tube for reverse transcription. |
Superior stability for heating
The amplification of 18S gene has a low efficiency at 37℃ and 42℃ respectively but if the temperature is raised up over 50℃, the amplification efficiency is remarkably improved because cDNA is optimally synthesized by raising the temperature of 18S gene, 18S rRNA and RNA themselves (1.8 kb).
Panel A : RevoScriptTMRT PreMix Kit. (Oligo dT)
Panel B : RevoScriptTMRT PreMix Kit. (Random)
Lane 1 : 200ng RNA template
Lane 2 : 20ng RNA template
Lane 3 : 2ng RNA template
Lane 4 : 0.2ng RNA template
Lane M : 1kb DNA Ladder
Ultra sensitive cDNA synthesis
Shows the high sensitivity of reverse transcription below 0.1pg.
Panel A : RevoScriptTMRT PreMix Kit. (Random)
Panel B : Supplier A Product
Panel C : Supplier B Product
Lane 1 : 575bp target gene
Lane 2 : 1kb target gene
Lane 3 : 1.6kb target gene
Lane 4 : 2.7kb target gene
Lane 5 : 4.5kb target gene
Lane 6 : 9kb target gene
Lane M : 1kb DNA Ladder
Suitable for the Full length cDNA
Possible for the cDNA synthesis above 9kb, shows the outstanding result compared with the competitor.
Lane M : Molecular weight marker
Lane 1 : 1ng RNA template
Lane 2 : 0.2ng RNA template
Lane 3 : 40pg RNA template
Lane 4 : 8pg RNA template
Lane 5 : 1.6pg RNA template
Lane 6 : 320fg RNA template
Lane 7 : 64fg RNA template | 
