Gel/PCR DNA Fragments Extraction Kit
This gel extraction/PCR cleanup kit, was designed to recover or concentrate DNA fragments (100 bp→10 Kb) from agarose gel, PCR, or other enzymatic reactions. Chaotropic salt is used to dissolve agarose gel and denature enzymes. DNA fragments in the chaotropic salt are bound by the glass fiber matrix of the spin column. Contaminants are removed with a Wash Buffer (containing ethanol) and the purified DNA fragments are eluted by a low salt Elution Buffer or TE. Salts, enzymes and unincorporated nucleotides can be effectively removed from the reaction mixture without phenol extraction or alcohol precipitation.
Specifications
- Sample: up to 300 mg (agarose gel slice)
up to 100 μl (PCR products)
- Format: spin column (centrifuge)
- Recovery: up to 90% (gel extraction), up to 95% (PCR cleanup)
- Operation time: 20 minute gel extraction, 15 minute PCR cleanup
- Applications: PCR, Fluorescent or Radioactive Sequencing,
Restriction Enzyme Digestion, DNA Labeling and
Ligation
- Storage: room temperature for up to 2 years
Gel Extraction PCR Cleanup
3,000bp
1,000bp
1 2 3
1. Geneaid
2. 1 Kb DNA Ladder
3. Competitor
|
1 2 3 4
| Lane |
Condition |
Yield |
260/280 |
260/230 |
| 1 |
Ladder |
|
|
|
| 2 |
Original |
/ |
/ |
/ |
| 3 |
Geneaid |
5.1 |
1.87 |
1.65 |
| 4 |
Competitor |
5 |
1.87 |
0.52 |
|
|
Gel/PCR DNA Fragments Extraction Kit
|
DF100/300
|
|
Gel/PCR DNA Fragments Extraction Maxi Kit
|
DM010/025
|
|
Small DNA Fragments Extraction Kit
|
DF101/301
|
|
Large DNA Fragments Extraction Kit
|
DF102/302
|
|
96-Well Gel/PCR DNA Extraction Kit
|
DFP04/10
|
Quick Protocol
DF Buffer reaction
of Gel slice or PCR product
DNA Binding
Wash
Elution |
