High-Speed Plasmid Mini Kit
The High-Speed Plasmid Mini Kit was designed for plasmid DNA purification or cosmid DNA purification from 1-4 ml of cultured bacterial cells. Modified Alkaline Lysis method and RNase treatment are used to obtain clear cell lysate with minimal genomic DNA/RNA contaminants. In the presence of chaotropic salt, plasmid DNA in the lysate binds to the glass fiber matrix of the spin column. Contaminants are removed with a Wash Buffer (containing ethanol) and the purified plasmid DNA is eluted by a low salt Elution Buffer or TE. Typical yields are 20-30 μg for high-copy number plasmid or 3-10 μg for low-copy number plasmid from 4 ml of cultured bacterial cells. DNA phenol extraction or alcohol precipitation is not required and the entire procedure can be completed within 30 minutes..
Specifications
- Sample: 1-4 ml of bacterial cells
- Format: spin column (centrifuge)
- Yield: up to 30 μg of plasmid DNA or cosmid DNA
- Applications: Restriction Enzyme Digestion,
Library Screening, Ligation, PCR,
Transformation/Sequencing Reactions
- Storage: room temperature for up to 2 years
- Operation time: within 30 minutes
Quick Protocol
Test Data
1 2 M 3 4 |
|
Brand Test
|
Test
|
260/280
|
260/230
|
Yield
|
|
Q
|
1
|
1.88
|
2.25
|
8.3 μg
|
|
2
|
1.88
|
2.24
|
8.4 μg
|
|
Geneaid
|
3
|
1.87
|
2.21
|
8.3 μg
|
|
4
|
1.89
|
2.23
|
8.5 μg
|
pBluescript (3 Kb), bacterial culture (1.5 ml)
Elution Volume: 50μl, M:1 Kb DNA Ladder
|
Click image to expand.
Sequencing Data
Click image to expand.
Various Plasmid Test Data
M1 1 2 3 4 5 6 M2
Lane 1: pBluescript II
Lane 2: pUC 18
Lane 3: pBR 322
Lane 4: pGEM with 1 Kb insert
Lane 5: pET 43.1 with 2.7 Kb insert
Lane 6: cosmid (50 Kb)
M1: 1 Kb DNA ladder, M2: Lambda-Hind III
|
|
