Viral Nucleic Acid Extraction Kit II
cell-free samples such as serum, plasma, body fluids and the supernatant of viral infected cell cultures (H1N1, HIV/AIDS, HPV, HBV, etc.). DNA/RNA viruses are lysed quickly and efficiently using the lysis buffer which is a highly concentrated solution of chaotropic salt. When combined with ethanol, the AD Buffer creates optimum conditions for Nucleic Acid binding to the glass fiber matrix of the column. Contaminants, such as salts, metabolites and soluble macromolecular cellular components are removed in the Wash step. Nucleic Acid is eluted in RNase-free water and is then ready for use in subsequent reactions.
Specifications
- Sample: 200 µl samples of plasma, serum, body fluids,
supernatant of viral infected cell cultures
- Format: spin column (centrifuge)
- Operation time: 40 minutes
- Applications: RT-PCR/PCR, Real-time PCR/Real-time RT-PCR,
Automated Fluorescent DNA Sequencing
- Storage: room temperature for up to 2 years
| Order Information |
Cat. Number |
| Viral Nucleic Acid Exraction Kit II (200 µl) |
VR050/100/300 |
| Viral Nucleic Acid Exraction Kit III (1 ml) |
VI050/100/300 |
| 96-Well Viral DNA/RNA Extraction Kit |
VNP04/10 |
Test Data
One-step Real-time Quantitative RT-PCR
Human beta-globin mRNA was detected in human total placental RNA samples ranging from 3 µg (E7 copies) to 0.3 pg (1 copy) using the ABI 7300 Sequence Detection
Dynamic range, sensitivity, and standard curve of the Viral DNA/RNA Extraction Kit
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Quantitation of viral DNA/RNA purified using the Viral DNA/RNA Extraction Kit
Real-time qPCR and 1-step qRT-PCR reactions were conducted to evaluate the performance of Geneaid’s Viral DNA/RNA Extraction Kit. HBV (DNA), HCV (RNA), HIV (RNA), and HTLV (RNA) were purified from 200 µl of positive clinical serum samples. The reactions were conducted using the ABI 7300 Sequence Detection System. Blood samples containing various amounts of DNA/RNA viruses ranging from 1 x E2 to 1 x E6 copies/ml were successfully detected and identified.
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Quick Protocol
Lysis
DNA/RNA
Binding
Wash
Elution
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