Genomic DNA extraction kit optimized for genomic DNA purification, mitochondrial DNA purification and virus DNA purification from a variety of animal tissue. The provided micropestle can efficiently homogenize tissue samples to shorten the time in the Lysis Step. Proteinase K and chaotropic salt are used to lyse cells and degrade protein, allowing DNA to be easily bound by the glass fiber matrix of the spin column. Once any contaminants have been removed, using a Wash Buffer (containing ethanol), the purified DNA is eluted by a low salt Elution Buffer or TE.
Specifications
- Sample: up to 30 mg of tissue
- Format: spin column (centrifuge)
- Yield: 10-20 µg (0.5 cm mouse tail), 20-50 µg (20 mg mouse kidney)
- Applications: PCR, AFLP/PADP, RFLP,
Southern Blotting, Real-time PCR
- Storage: room temperature for up to 2 years
- Operation time: within 1 hour
Quick Protocol
Test Data
Various genomic DNA was purified using
Geneaid’s Genomic DNA Mini Kit (Tissue).
Right lane: Purified gDNA digested with EcoRI,
M: 1 Kb DNA Ladder
| Mouse Sample |
Size |
Yield (μg) |
| Tail |
0.5 cm |
10-20 |
| Liver |
20 mg |
10-20 |
| Brain |
20 mg |
10-20 |
| Lung |
20 mg |
5-10 |
| Muscle |
20 mg |
5-10 |
| Kidney |
20 mg |
20-50 |
