Total RNA Mini Kit (Blood/Cultured Cell)
The Total RNA Purification Mini Kit (Blood/Cultured Cell) was designed specifically for total RNA
purification from fresh whole human blood and cultured cells. Detergents and chaotropic salt are used to lyse cells and inactivate RNase (optional DNase treatments can be followed to remove unwanted DNA residue). RNA in the chaotropic salt is bound by the glass fiber matrix of the spin column and once any contaminants have been removed, using the Wash Buffer (containing ethanol), the purified total RNA is eluted by RNase-free water.
Specifications
- Sample: up to 300 µl of whole blood,
cultured cells (up to 5 x 106)
bacterial cells (up to 1 x 109)
fungus cells (up to 5 x 107)
- Format: spin column (centrifuge)
- Yield: 5-30 μg
- Operation time: within 1 hour
- Applications: RT-PCR, Northern Blotting, Primer
Extension, mRNA Selection and cDNA Synthesis
| Order Information |
Cat. Number |
| Total RNA Mini Kit (Blood/Cultured Cell) |
RB050/100/300 |
| Total RNA Mini Kit (Tissue) |
RT050/100/300 |
| Total RNA Mini Kit (Plant) |
RP050/100/300 |
| Total RNA Maxi Kit (Blood/Cultured Cell) |
RBM10/25 |
| Total RNA Maxi Kit (Tissue) |
RTM10/25 |
| Total RNA Maxi Kit (Plant) |
RPM10/25 |
| 96-Well Total RNA Kit |
RBP04/10 |
| miRNA Isolation Kit |
PU009100 |
Buffers are also availabe separately
W1000045 W1 Buffer 45 ml $30.00
W1000130 W1 Buffer 130 ml $54.00
WB000025 Wash Buffer 25 ml $12.00
WB000050 Wash Buffer 50 ml $18.00
EB000006 Elution Buffer 6 ml $6.00
EB000030 Elution Buffer 30 ml $12.00
RBC00060 RBC Lysis Buffer 60 ml $30.00
RBC00135 RBC Lysis Buffer 135 ml $45.00
RB000030 RB Buffer 30 ml $45.00
RB000060 RB Buffer 60 ml $75.00
RB000130 RB Buffer 130 ml $135.00 | 
