SensiFAST SYBR No-ROX Kit

SensiFAST SYBR No-ROX Kit

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SensiMix II Probe Kit

SensiMix II Probe Kit

SensiFAST SYBR No-ROX Kit

SensiFAST SYBR No-ROX Kit

SensiFAST SYBR No-ROX Kit

Outstanding assay reproducibility, sensitivity and robustness from both DNA and RNA templates, under fast thermal cycling conditions.

CA$275.00
SKU
BIO-98005

Description

Product Highlights

  • Reproducible consistent results between technical replicates for increased confidence in results
  • Specific antibody-mediated hot-start DNA polymerase minimizes non-specific amplification for improved assay sensitivity and reliability
  • Sensitive reliable quantification of low abundance targets and scarce samples
  • Robust reliable, accurate detection of DNA and RNA targets from a broad range of sample types
  • Fast delivers reproducible, accurate assay results in as little as 30 minutes

Product Description

The SensiFAST SYBR No-ROX Kit has been developed for fast, highly accurate real-time PCR and has been validated on all commonly-used real-time instruments that do not require the passive reference dye ROX.

A combination of the latest advances in buffer chemistry and PCR enhancers ensures that the SensiFAST SYBR No-ROX Kit produces reliable assay results under fast thermal cycling conditions.‚  An antibody-mediated hot-start DNA polymerase system promotes highly-specific amplification, in turn improving assay sensitivity and dynamic range.‚ 

The SensiFAST SYBR®‚ No-ROX Kit has been optimized to deliver optimal performance in tandem with the‚ SensiFAST cDNA Synthesis Kit, which offers fast, unbiased cDNA synthesis, without compromising cDNA yield or coverage

 

Applications

  • Gene expression analysis
  • DNA / RNA target detection
  • miRNA profiling / quantification
  • Copy number variation (CNV) analysis

Specifications

Components

Reagent

     200 x 20µl Reactions     

     500 x 20µl Reactions     

     2000 x 20µl Reactions     

SensiFAST SYBR® No-ROX mix (2x)

2 x 1ml

5 x 1ml

4 x 5ml

 

 

Volume

  • BIO-98002: 250 x 20µl        Reactions: 2 x 1ml
  • BIO-98005: 500 x 20µl        Reactions: 5 x 1ml
  • BIO-98020: 2000 x 20µl      Reactions: 4 x 5ml

 

Storage & Stability

All kit components should be stored at -20°C upon receipt for optimum stability. Repeated freeze/thaw cycles should be avoided. Avoid exposure of the SYBR® Green I to light.

When stored under the recommended conditions and handled correctly, full activity of the kit is retained until the expiry date indicated on the outer box label.

 

Shipping conditions

SensiFAST SYBR® No-ROX Kit is shipped on dry/blue ice

Video

Brochures

BIO-98005 brochure