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HIGHDEF(R)DAB Chromogen/Substrate Set
IHC/ISH Peroxidase Block
HIGHDEF(R) Hematoxylin
Ready-to-use histologic stain with enhancers
Protocol
- Deparaffinize and rehydrate tissue.
- Apply enough HIGHDEF® Hematoxylin to completely cover the tissue (usually about 150 μL) and incubate for 2-10 minutes, according to desired intensity.
- Rinse in deionized water for 20 to 30 seconds.
- Follow suggested dehydration and mounting procedures based on the chromogen used.
