Nutristem MSC XF Basal Medium

Nutristem MSC XF Basal Medium

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Nutristem MSC XF Supplement Mix

Nutristem MSC XF Supplement Mix

Nutristem MSC XF Basal Medium

Nutristem MSC XF Basal Medium

Nutristem MSC XF Basal Medium

Designed for optimal growth and expansion of human MSCs. Defined, xeno-free, serum-free medium

CA$155.00
SKU
05-200-1A

Description

Faster growth rates and stronger cell yields

MSC NutriStem® XF Medium is a defined, serum-free, xeno-free culture medium designed for optimal growth and expansion of human mesenchymal stem/stromal cells (hMSC) derived from a variety of sources, including bone marrow (BM-MSC), adipose tissue (AT-MSC) and umbilical cord matrix (UC-MSC). MSC NutriStem® XF Medium supports long-term growth of hMSC while maintaining their self-renewal and multi-lineage differentiation potential.

hMSCs cultured in MSC NutriStem XF Medium show superior proliferation and self-renewal potential in comparison to serum-containing media and other commercial serum-free media. In addition, hMSCs maintain their proper fibroblast-like cell morphology, tri-lineage differentiation potential, and demonstrate normal hMSC marker profiles, and karyotypic stability over long-term culture.

Key advantages

  • Serum-free, xeno-free, defined culture system
  • Superior cell growth compared to serum-containing media
  • Supports long-term growth and self-renewal of hMSCs from multiple sources
  • Consistent, reliable MSC cultures
  • Suitable for the harvest of high quality exosomes (EVs) for future clinical applications
  • Eliminates the need to spend time and money pre-qualifying FBS lots
  • Manufactured under cGMP
  • FDA Drug Master File (DMF) available

Cells cultured in serum-containing medium do not require an adaptation phase when transitioning to serum-free MSC NutriStem® XF Medium.

Sample Data

Initial isolation

Isolation

Figure: Human mesenchymal stem cells were initially isolated from Wharton's jelly (left image) and placenta (right image) using MSC NutriStem XF on pre-coated plates with MSC Attachment Solution (cat. no. 05-752-1). High number of hMSC was obtained.

Proliferation

Proliferation

Figure: MSC NutriStem® XF promotes excellent proliferation of hMSC from a variety of sources while maintaining normal fibroblast-like, spindle shape cell morphology.

Self-renewal potential

Self-renewal potential

Figure: hMSC-BM and hMSC-AT were expanded in MSC NutriStem® XF for 3-5 passages prior to 14 days of CFU-F assay. Colonies were stained with 0.5% crystal violet (x100). The cells maintain their self-renewal potential.

Differentiation potential

Differentiation potential

Figure: hMSC-BM (top panel) and hMSC-AT (bottom panel) expanded in MSC NutriStem® XF for 3-5 passages prior to trilineage differentiation. Representative images of stained adipocytes (Oil Red O), osteocytes (Alizarin Red) and chondrocytes (Alcian Blue). The cells maintain their multilineage differentiation potential.

Growth curve

growth

Figure: NutriStem® MSC XF Medium promotes superior proliferation and expansion of hMSCs over time as compared to other serum-free and serum-containing media.

Specifications

MSC NutriStem® XF Basal Medium (Cat.#05-200-1A and Cat.#05-200-1B) should be stored at 2-8°C. 

The complete MSC NutriStem® XF culture medium is stable at 2-8°C for up to 30 days. Protect from light.