A unique combination of PCR products and a number of proprietary plasmids digested with appropriate restriction enzymes to yield 11 fragments, suitable for use as molecular weight standards for agarose gel electrophoresis. The DNA includes fragments ranging from 100-1,500 base pairs. The 500 and 1,500 base pair bands have increased intensity to serve as reference points. The pproximate mass of DNA in each band is provided (0.5 μg a load) for approximating the mass of DNA in comparably intense samples of similar size.
PCR products and double-stranded DNA digested with appropriate restriction enzymes, are phenol extracted and equilibrated to 10 mM Tris-HCl (pH 8.0) and 10mM EDTA.
Range: 100-1,500 bp Number of bands: 11 Concentration: 100 μg/ml Package: 50ug/500ul Recommended Load: 5 μl / well
Containing orange G & xylene cyanol FF as tracking dyes.
Negative 20 C
Store at 25°C for 6 months
Store at 4°C for 12months
Store at -20°C for 24 months