Welcome to FroggaBio
Toll-Free: 1-877-318-7277

Presto™ Midi Plasmid Kit

Presto™ Midi Plasmid Kit

Promo: For every 3 you order, we will add 1 more FREE!

US$199.00
Cat# PIF025
Size 25 preps
Protocol

Description

    The Presto™ Midi Plasmid Kit was designed for Plasmid DNA Purification from 50-150 ml of cultured bacterial cells using an efficient plasmid midiprep system. For processing smaller volumes, the Presto™ Mini Plasmid Kit is also available. Both plasmid kits include TrueBlue Lysis Buffer (an optional color indicator) to prevent common handling errors, while ensuring efficient cell lysis and neutralization. The Presto™ Midi Plasmid Kit uses a modified alkaline lysis method and RNase treatment  to obtain clear cell lysate with minimal genomic DNA/RNA contaminants. Using an efficient gravity-flow procedure, plasmid DNA in the crude lysate is bound by the plasmid midi column and the contaminants are removed with PW Buffer. The purified plasmid DNA is eluted by a high salt buffer and then precipitated with Isopropanol for desalting. Due to the high efficiency of the new Presto Midi Spin Column, the entire procedure can be completed in 80 minutes without ultracentrifuges, HPLC or other toxic reagents.  

    Advantages (Cat. # PIF025)
    • High Yield: >400 µg of pure transfection grade plasmid DNA from 100 ml of cultured bacterial cells
    • Convenient: TrueBlue Lysis Buffer is an optional color indicator included with this plasmid kit in order to reduce common handling errors, while ensuring efficient cell lysis and neutralization and maximum plasmid product yield so you can feel confident with each and every plasmid prep.
    • Sample Volume: 50-150 ml of cultured bacterial cells
    • Format: Plasmid Midi Anion-Exchange Resin Columns and Presto™ Midi Spin Columns (membrane optimized for plasmid DNA purification)
    • Storage: dry at room temperature (15-25°C) for up to 2 years
    • Operation time: within 80 minutes (only 40 minutes when combined with the Presto™ Plasmid Concentrator)
    Applications
     
    Transfection, Sequencing, Ligation, PCR, In-vitro Transcription, Microinjection, Restriction Enzyme Digestion, Gene Gun
     
    Components
    • PM1, PM2, PM3 Buffers
    • TrueBlue Lysis Buffer
    • PEQ Buffer
    • PW Buffer
    • PEL Buffer
    • RNase A
    • Presto™ Midi Columns (yellow spin columns)
    • Plasmid Midi Columns (anion exhange white resin columns)
    Quality Control
     
    The quality of the Presto™ Midi Plasmid Kit is tested on a lot-to-lot basis according to Geneaid's ISO-certified quality management system. Plasmid DNA from a 50 ml overnight E. coli (DH5α) culture, containing plasmid pBluescript (A600 > 2 U/ml) is purified using the Presto™ Midi Plasmid Kit. Following the purification process, a yield of more than 200 μg is obtained and the A260/A280 ratio is between 1.8-2.0. The purified plasmid (1 µg) is used in EcoRI digestion, and analyzed by electrophoresis.
     
     
     
     
     
Specification

    Format: Anion-Exchange Gravity Flow

    Binding Capacity: 500 µg

    Presto™ Column: Yes

    Endotoxin Level: <0.15 EU/µg

    Culture Input: 50-150 ml

    Plasmid Size: 1-20 kb

    Typical Yield: 200-500 µg

    Elution Volume: 500 µl-2 ml

    Operation Time: <80 minutes