RedSafe™ Nucleic Acid Staining Solution (20,000x) is a new and safe nucleic acid staining solution. It is an alternative to the traditional ethidium bromide(EtBr) stain for detecting nucleic acid in agarose gels. It emits green fluorescence when bound to DNA or RNA. This new stain has two fluorescence excitation maxima when bound to nucleic acid, one caentered at 309 nm and another at 419 nm. In addition, it has one visible excitation at 514 nm. The fluorescence emission of RedSafe™ bound to DNA is centered at 537 nm. RedSafeTM Nucleic Acid Staining Solution (20,000x) is as sensitive as EtBr. The protocol is also similar with a protocol of EtBr. The Ames test proves lower mutation with RedSafe™ Nucleic Acid Staining solution compare with EtBr. In addition, RedSafe™ Nucleic Acid Staining Solution (20,000x) has a negative result in mouse marrow chromophilous erythrocyte micronucleus test and mouse spermary spermatocyte chromosomal aberration test.
Therefore, it is wise to choose RedSafe™ Nucleic acid Stainig Solution (20,000x) instead of EtBr for detecting nucleic acid in agarose gels.