Count Cells Without Slides
Watch the video to see how much plastic waste has been reduced by scientists using CellDrop Automated Cell Counters around the world.
Count Cells Without Slides
Reduce plastic waste, eliminate disposable slide costs, and automate cell counting in your lab with DeNovix CellDrop Automated Cell Counters.
Why Choose CellDrop?
DeNovix patented DirectPipette™ technology replaces hemocytometers and plastic slides traditionally used in cell counting equipment. The CellDrop is the only automated cell counter that eliminates the costs and plastic waste associated with cell counting slides.
Count Cells Without Slides
Automate counts, eliminate costs, and reduce plastic waste in your cell counting workflow.
Rapid Cell Counts & Viability
Brightfield – 3 seconds
Dual Fluorescence – 8.5 seconds
Maintenance-Free Design
No recalibration required for the life of the instrument.
DirectPipette™ Technology
Trust in the reproducibility of your results with CellDrop’s patented sample chamber design.
How it Works: The Sample Chamber
- Two permanent, optical grade sapphire surfaces
- Sample is held in place by surface tension
- Three software-selectable chamber heights enable:
- Widest dynamic range: 7 x 102 – 2.5 x 107 cells/mL
- Cell size range: 4 – 400 µm
1. Load Sample
Load cell suspension into sample chamber. Sample can be loaded from either side of the arm.
2. Count & Analyze
Press the Count button, and let the CellDrop do the work! Count data will appear in 3–8 seconds.
3. Wipe Clean
After wiping the surfaces with a dry lab wipe, the CellDrop is ready for the next sample.
Wide Variety of Applications
The CellDrop software excels in a wide variety of applications. View CellDrop technical notes to learn more.
Primary Cells
Tissue Culture
Isolated Nuclei
GFP
Yeast
Protoplasts
Pollen
Algae Cells
Insect Cells
Reducing Plastic Waste in Cell Counting
The CellDrop’s innovative design improves efficiency and sustainability in cell counting equipment by removing the need for plastic slides. Scientists around the world have prevented millions of plastic slides from being disposed of by counting cells without slides.
First ACT® Label Certified Automated Cell Counter
The CellDrop has achieved ACT® Label certification from My Green Lab®, making it the first automated cell counter accredited by the program. The ACT Environmental Impact Factor Label provides a third-party verification for the environmental impact of laboratory products.
How Much Would Your Lab Save with CellDrop?
No slides = less plastic waste and less money spent on consumables.
Models: Automated Brightfield and Fluorescence
CellDrop BF
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Brightfield
Rapid and reliable for tissue culture counts and viability measurements. Widely used for samples with low or no debris present.
Brightfield, Trypan Blue, Primary Trypan Blue, Nuclei Trypan Blue
CellDrop FL
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Dual Fluorescence & Brightfield
Improve the accuracy of counts and viability measurements, even with challenging samples. Dual fluorescence optics and assays such as AO/PI (Acridine Orange / Propidium Iodide) combine to eliminate subjectivity and reliably identify live and dead cells.
Brightfield, Trypan Blue, Primary Trypan Blue, AO/PI, AO, PI, Primary AO/PI, GFP, Yeast, Custom Apps, Nuclei Trypan Blue, Nuclei AO/PI
A Splash of Color in Your Lab
CellDrop is available in two colors: Leaf Green and Arctic White.
Powerful, Intuitive Software
Results
CellDrop results include a wide range of application-specific metrics for each cell sample. Data is clearly displayed on-screen and auto-saved to a large onboard hard drive. Metrics include:
Graphical Data Display
A wide range of graph types are available to gain additional sample insight. Overlay multiple samples from the same session or from the data archive for powerful data visualization.
Dilution Calculator
The convenient dilution calculator automatically calculates target dilution volumes of cell culture and buffer based on each cell count. Dilution guidance is displayed prominently on-screen and in printed reports for easy sample processing.
EasyApps™ Software
CellDrop EasyApps software is designed by life scientists, for life scientists. The system features a high definition, 7″ touchscreen and pre-installed apps for a range of common applications.
Additional apps for accounts, data, and other utilities contribute to the system’s easy-to-use interface.
Optimized Measurement Apps
DeNovix CellDrop Series instruments are stand-alone systems equipped with intuitive, easy-to-use apps for automatic cell counting and viability analysis.
Brightfield
- Count brightfield cell images in ~3 seconds
- Live/dead assessment using Trypan Blue or Erythrosin B
- Cells/mL, cell size, and % viability reported
- Nuclei concentration and extraction efficiency using Trypan Blue
Fluorescence
- Cell viability analysis using Acridine Orange (AO) and Propidium Iodide (PI) in ~8.5 seconds
- Analyze primary cells in the presence of debris
- Nuclei concentration and extraction efficiency using AO/PI assay
- Yeast viability
- GFP transfection efficiency
- Custom methods for other applications
Data App & Analysis
- All results and images are automatically saved and searchable in the Data App
- Large onboard memory enables storage and retrieval of thousands of results
- Analyze and optimize previous counts
- Easily export results and graphs
Exporting
Export results and images to USB, email, network folders, or printers. Export formats include PDF reports, .csv files, and .png files.
Counting Protocols
- Use built-in default protocols, or customize to streamline your workflow
- Create, save, and easily optimize protocols
- Custom settings include:
- Dilution factor
- Minimum / maximum diameter
- Cell / nuclei roundness
- Live / dead intensity threshold
- Sample mode (DirectPipette or Slide)
- Irregular cell mode
- Three chamber height options (400 µm, 100 µm, or 50 µm)
Protocol Settings Database
- CellDrop protocol settings for cells, primary cells, and nuclei
- Use as a starting point for your sample, or reach out to our applications team
Controls Toolbar
- Name sample
- Choose protocol
- Exposure
- Focus (includes Autofocus)
- Fluorescence channel selector
- Dilution calculator
Autofocus
Setting the focus on the CellDrop is easy. Because of the CellDrop’s fixed sample surfaces, adjusting focus is not often needed once it has been set. However, the software is also equipped with Autofocus. Just press the Auto button in the focus menu to automatically adjust to the correct focus.
Cell Size Gating
Cell / Nuclei Size Histograms allow easy gating for non-target cells or cell debris. Simply drag the slider to set the cell size of interest and view the results in real time. Results are instantly re-calculated excluding all debris or cells that fall outside of the defined size range
GFP Intensity Gating
When using the dedicated GFP App, users can gate cells by fluorescence intensity as well as size. The intensity histogram displays the number of cells counted at each GFP intensity level allowing the exclusion of non-GFP expressing cells or low expressing cells to be excluded from analysis.
Advanced Declustering Algorithms
All CellDrop Count Apps use proprietary declustering algorithms developed by DeNovix. Complex samples of regular or irregular cells over a wide range of cell density can be rapidly and accurately counted. Analysis of cluster size and frequency can be performed and graphically displayed.
Irregular Cell Mode
For cells that are elongated or irregularly shaped, enable Irregular Cell Mode from the Protocol Settings or the Optimize Setting button on the results screen. This is ideal for cells that are not round when trypsinized, such as fibroblasts or epithelial cells with a high degree of structure.
Irregular Cell Mode is also used to identify irregularly shaped isolated nuclei, such as smooth muscle nuclei from heart tissue.
IQ/OQ
A full installation and qualification (IQ/OQ) package is available for the CellDrop Automated Cell Counter.
Performance Verification Slide
The performance verification slide is a slide with beads fixed on the surface and is designed for assessing the accuracy and precision of a CellDrop Automated Cell Counter. Each slide includes a factory-certified value for the number of beads on the slide.