ExoQuick TC 10ml

 Need exosomes? SBI’s ExoQuick-TC enables high-throughput, quantitative isolation of exosomes from low volumes (as little as 1 ml) of tissue culture media and certain biofluids (saliva, urine, follicular fluid, and breast milk). Note, to isolate exosomes from serum, plasma, or ascites fluid, use the original ExoQuick formulation (EXOQ5A-1 or EXOQ20A-1)). Compatible with a wide variety of downstream applications, ExoQuick-TC is an effective and proven alternative to ultracentrifugation^1-3.

ExoQuick-TC’s fast, ultracentrifugation-free method:

• Saves time and labor
• Is easily scalable
• Conserves precious sample
• Delivers high yields of functional, high quality exosomes
• Can be used to isolate exosomes for a wide range of downstream applications, including
  • Biomarker studies
  • Exosomal miRNA profiling
  • Exosomal proteomics
  • Exosomal lipidomics/metabolomics
  • Functional studies, such as in cell-to-cell signaling
  • Basic biology, such as role in tumorigenesis

ExoQuick-TC is a proprietary polymer that gently precipitates exosomes. First, pre-clear your samples of cells and cellular debris, and then simply add the appropriate amount of ExoQuick-TC to your cleared biofluid, refrigerate, and centrifuge (see the product manual for protocol details). Your exosomes will be in the pellet, ready for resuspension in an appropriate solution.

How It Works: High-throughput, quantitative exosome recovery

ExoQuick-TC can be used to purify exosomes from a wide variety of tissue culture media⁴, and from certain biofluids such as saliva¹, urine², follicular fluid³, and breast milk⁵. With a simple workflow involving minimal hands-on time and low input sample volume requirements, ExoQuick-TC is an excellent option for researchers who need to purify multiple exosome samples.

To isolate exosomes from tissue culture media, simply:

• Add an appropriate volume of ExoQuick-TC
• Incubate overnight at 4°C
• Isolate exosomes with a 30-minute low-speed spin (1500g).

Isolated exosomes can be found in the pellet and resuspended in an appropriate solution.

With ExoQuick-TC, you can obtain high-quality exosomes from tissue culture media and certain biofluids using a protocol that can easily be performed on multiple samples and requires very low volumes of input sample