Small DNA Fragments Extraction Kit
The Small DNA Fragments Extraction Kit is a PCR Cleanup Kit and Gel Extraction Kit in 1 convenient system optimized to recover or concentrate 40-200 bp DNA fragments from agarose gel, PCR, or other enzymatic reactions. For processing 70 bp-20 kb DNA fragments, the Gel/PCR DNA Fragments Extraction Kit is available. For processing 10-50 kb DNA fragments, the Presto™ Max Gel/PCR Kit is also available. Chaotropic salt is used to dissolve agarose gel and denature enzymes. DNA fragments in the chaotropic salt are bound by the glass fiber matrix of the spin column. Contaminants are removed with a Wash Buffer (containing ethanol) and the purified DNA fragments are eluted by a low salt Elution Buffer, TE or water. Salts, enzymes and unincorporated nucleotides can be effectively removed from the reaction mixture without phenol extraction or alcohol precipitation. Typical recovery is up to 90% for Gel Extraction and up to 95% for PCR Cleanup. The eluted DNA is ready for use in PCR, Fluorescent or Radioactive Sequencing, Restriction Enzyme Digestion, DNA Labeling and Ligation.
Advantages (Cat. # DF101, DF301)
- Purify small DNA fragments within 10 minutes!
- Up to 95% recovery of small DNA fragments
- Optimized for 40 bp–200 bp DNA fragment recovery
- Sample Size: up to 300 mg (agarose gel slice), up to 100 μl (PCR products)
- Gel/PCR glass fiber spin column (green membrane optimized for both gel extraction and PCR cleanup of small DNA fragments)
- Storage: room temperature for up to 2 years
- SD Buffer
- W1 Buffer
- Wash Buffer
- Elution Buffer (10 mM Tris-HCl, pH8.5 at 25°C)
- SD Columns
- 2 ml Collection Tubes
Quality Control
The quality of the Small DNA Fragments Extraction Kit is tested on a lot-to-lot basis according to Geneaid's ISO-certified quality management system. DNA fragments are purified from either agarose gel, PCR products or other aqueous solutions and analyzed by electrophoresis.
