Presto Mini gDNA Bacteria Kit (w/Lysozyme)
The Presto™ Mini gDNA Bacteria Kit is a genomic DNA extraction kit optimized for genomic and viral DNA purification from Gram negative bacterial cells and Gram positive bacterial cells. ThisDNA Extraction Kit includes Gram+ Buffer, which combined with lysozyme, will efficiently lyse bacterial cell walls consisting of the peptidoglycan layer.Chaotropic salt is used to lyse cells and degrade protein, allowing DNA to easily bind to the glass fiber matrix of the genomic DNA spin column. Contaminants are removed using a Wash Buffer (containing ethanol) and the purified genomic DNA is eluted by a low salt Elution Buffer, TE or water. The entire procedure can be completed within 20 minutes without phenol/chloroform extraction or alcohol precipitation. The purified DNA, with approximately 20-40 kb, is suitable for use in PCR or other enzymatic reactions.
- Purify genomic DNA within 20 minutes!
- High Yield: 25-30 μg (1 x 109 Escherichia coli) 10-15 µg (1 x 109 Bacillus subtilis)
- High Purity gDNA: A260/A280 >1.9
- DNA Size: 20-40 kb
- Wide Variety of Bacteria Sample Strains: Gram (+) Positive and Gram (-) Negative bacterial cells (up to 1 x 109)
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Convenient: Includes Gram+ Buffer for preparing lysozyme solutions to speed up sample preparation when purifying DNA from Gram (+) positive bacteria
- Genomic DNA glass fiber spin column (green membrane optimized for genomic DNA purification from bacteria)
- Elution volume: 30-200 µl
- Storage: dry at room temperature (15-25°C) for up to 1 year
- Gram+ Buffer
- GT Buffer
- GB Buffer
- W1 Buffer
- Wash Buffer
- Elution Buffer (10 mM Tris-HCl, pH8.5 at 25°C)
- GD Columns
- 2 ml Collection Tubes
Quality Control
