GENEzol TriRNA Bacteria Kit
US$440.44
SKU
GZB050
Size
50 rxns
GENEzol™ TriRNA Bacteria Kit is a phenol, chloroform and guanidine isothiocyanate based scalable solution for extracting high-quality total RNA as well as simultaneous RNA, DNA and protein extraction from bacteria samples. The provided Lysozyme and Bacteria Lysis Buffer will efficiently lyse bacterial cell walls consisting of the peptidoglycan layer. The extracted RNA can be used directly in a variety of downstream applications.
Advantages (Cat. # GZB050, GZB100)
- Extract total RNA or simultaneous RNA, DNA and protein within 1 hour
- Sample Volume: bacteria cells up to 1 x 109
- A cost effective, scalable solution
- Format: phenol, chlorform, guanidine isothiocyanate
- Storage: GENEzol™ Reagent should be stored at 2ºC to 25ºC for up to 9 months, Bacteria Lysis Buffer should be stored at room temperature (15-25ºC) for up to 9 months, Lysozyme should be stored at -20ºC for extended periods
Applications
cDNA Library Construction, Cloning, RT-PCR (Endpoint), Real-Time PCR, Nuclease Protection Assays, Northern Blotting
Components
- GENEzol™ Reagent
- Bacteria Lysis Buffer
- Lysozyme
Quality Control
GENEzol™ TriRNA Bacteria Kit is tested on a lot-to-lot basis according to Geneaid’s ISO-certified quality management system. AnEscherichia coli (1 × 109) culture (OD600=1.3, 1 ml) is harvested by centrifugation at 16,000 x g for 2 minutes, followed by RNA extraction. 10 µl from a 50 µl eluate of RNA is analyzed by electrophoresis on a 0.8% agarose gel.
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GENEzol TriRNA Bacteria Kit
US$800.80
