GenepHlow Gel/PCR Kit
The GenepHlow™ Gel Extraction Kit and PCR Cleanup Kit was designed to recover or concentrate DNA fragments from agarose gel, PCR or other enzymatic reactions. This gel extraction kit and PCR cleanup kit includes a pH indicator which is premixed with the binding buffer to ensure optimal pH, facilitate DNA binding and allow for easy observation of undissolved agarose gel. If pH exceeds the optimal level (>7.5), the color of the solution will appear purple instead of yellow. 3M Sodium Acetate (pH5.0), which is included with the kit, can then be added to the solution to adjust pH and return the color to yellow. Chaotropic salt is used to dissolve agarose gel and denature enzymes. DNA fragments in the chaotropic salt are bound by the glass fiber matrix of the spin column. Contaminants are removed with a Wash Buffer (containing ethanol) and the purified DNA fragments are eluted by a low salt Elution Buffer, TE or water. The pH indicator, salts, enzymes and unincorporated nucleotides can be effectively removed from the reaction mixture without phenol extraction or alcohol precipitation and the purified DNA is ready for use in subsequent reactions.
Advantages (Cat. # DFH100, DFH300)
- High Efficiency: up to 90% recovery from agarose gels, up to 95% recovery from PCR or other enzymatic reactions
- Only 20 minute gel extraction and 10 minute PCR cleanup
- Convenient: Gel/PCR binding buffer is premixed with a pH indicator to allow for easy determination of optimal pH
- Includes 3M Sodium Acetate (pH5.0) to adjust pH if it becomes too high
- Sample Size: up to 300 mg of agarose gel slice or up to 100 μl of PCR products
- Broad Fragment Size Range: 70 bp–20 kb
- Gel/PCR glass fiber spin columns (blue membrane optimized for both gel extraction and PCR cleanup)
- Storage: dry at room temperature (15-25°C) for up to 1 year
Applications
Fluorescent and Radioactive Sequencing, PCR, Restriction Enzyme Digestion, DNA Labeling and Ligation, Microarray, Transfection
Components
- Gel/PCR Buffer (premixed with a pH indicator)
- 3M Sodium Acetate (pH5.0)
- W1 Buffer
- Wash Bufffer
- Elution Buffer (10 mM Tris-HCl, pH8.5 at 25ºC)
- DFH Columns
- 2 ml Collection Tubes
