GENEzol Reagent
US$600.60
SKU
GZR200
Size
200 ml
GENEzol™ Reagent is a phenol, chloroform and guanidine isothiocyanate based scalable solution for extracting high-quality total RNA as well as simultaneous extraction of RNA, DNA and protein from a wide variety of samples such as blood, buffy coat, plasma, serum, cultured cells and tissue. The extracted RNA can be used directly in a variety of downstream applications such as cDNA Library Construction, Cloning, RT-PCR (Endpoint), Real-Time PCR, Nuclease Protection Assays and Northern Blotting. GENEzol™ Reagent is a cost effective alternative for efficient total RNA extraction.
Advantages (Cat. # GZR050, GZR100, GZR200)
- Extract total RNA or simultaneous RNA, DNA and protein within 1 hour
- Sample Volume: up to 300 μl of blood, buffy coat, serum, plasma, up to 5 x 106 cultured cells, 50-100 mg of tissue
- A cost effective, scalable solution
- Format: phenol, chlorform, guanidine isothiocyanate
- Storage: 2ºC to 25ºC for up to 9 months
Applications
cDNA Library Construction, Cloning, RT-PCR (Endpoint), Real-Time PCR, Nuclease Protection Assays, Northern Blotting
Components
- GENEzol™ Reagent
Quality Control
GENEzol™ Reagent is tested on a lot-to-lot basis according to Geneaid’s ISO-certified quality management system. RNA from a 1 ml human blood sample is extracted using GENEzol™ Reagent. 10 µl from a 50 µl eluate of RNA is analyzed by electrophoresis on a 0.8% agarose gel.
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