IRIS11 Prestained Protein Ladder
This product can be purchased in the USA exclusively through Gemini-Bio
Click link to buy PMI0500, PMI11-0500K, PMI11-0500M, PMI11-0500G
The IRIS11 Prestained Protein Ladder is a combination of 11 pre-stained proteins with molecular weights from 3 to 260 kDa. The 11 recombinant proteins are covalently coupled with blue chromophore, while 2 red bands at 70 kDa and 260 kDa, a green band at 15 kDa and a newly designed peacock green band at 60 kDa serve as reference bands. The IRIS11 Prestained Protein Ladder keeps track of the size and separation of proteins during SDS-polyacrylamide gel electrophoresis, approximating the target protein size and validating the Western transfer efficiency on PVDF, nylon, or nitrocellulose membranes.
Specifications
| Product Name: | IRIS11 Prestained Protein Ladder | Catalog Number: | PMI11-0500 |
| Molecular Weight Range: | 3 to 260 kDa | Reference Bands: | 11 |
| Color: | 4-color (Blue, Green, Red, Peacock green) | Protein Concentration: | Approximately 0.2~0.4 mg/ml of each protein |
| Prestained: | Yes | Ready To Use: | Yes |
| Recommended Gel: | SDS-PAGE (Tris-glycine buffer) | Package: | 500μl x 1 vial |
| Loading Buffer: | 20 mM Tris-phosphate (pH 7.5 at 25°C), 2 % SDS, 0.2 mM Dithiothreitol, 3.6 M Urea, and 15 % (v/v) Glycerol |
Recommended Loading Volume: | 3µl per well (15well) for electrophoresis 5µl per well (10well) for electrophoresis 1.5 - 2.5µl per well for western blotting |
| Number Of Applications: | Sufficient for 100 applications (5μl per loading) Sufficient for 165 applications (3μl per loading) |
Storage: | Stable for up to 2 weeks at 25°C Stable for up to 3 months at 4°C For long term storage, store at -20°C |
Migration patterns of IRIS11 Prestained Protein Ladder in various electrophoresis conditions:
Note: The molecular weight of each protein (kDa) was measured against an unstained protein ladder in every electrophoresis condition. Additional data should be considered for a more accurate adjustment.
- 3 μl or 5 μl per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively
- 2.5 μl per well for general Western transferring
- Apply more for thicker (> 1.5 mm) or larger gel
All products are for research use only.
More To Explore:
Transfer your target proteins and protein ladder to a Western Blot membrane for detection with our chemiluminescent substrates .
PDF's
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IRIS11-Protocol.pdf Size: (19.53 KB)
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IRIS11-SDS.pdf Size: (19.53 KB)
Starting at US$129.00
