Presto 96 Well Plant Genomic DNA Extraction Kit
The Presto™ 96 Well Plant Genomic DNA Extraction Kit is designed for high-throughput purification of total DNA (including genomic DNA, mitochondrial and chloroplast DNA) from various plant species. Homogenized samples are treated with RNase A then centrifuged to remove cell debris and salt precipitates. In the presence of a binding buffer, genomic DNA in the lysate binds to the glass fiber matrix of the Presto™ 96 Well gDNA Binding Plate. Contaminants are removed using a Wash Buffer and the purified genomic DNA is eluted by a low salt Elution Buffer, TE or water. The entire procedure can be completed within 60 minutes without phenol/chloroform extraction or alcohol precipitation. The purified DNA (approximately 20-30 kb) is suitable for use in PCR or other enzymatic reactions.
Advantages (Cat. # 96GPP04, 96GPP10)
- Purify 96 wells of pure genomic DNA from a variety of plant species in 60 minutes!
- Sample Volume: 100 mg of fresh plant tissue and 25 mg of dried plant tissue per well
- Yield: up to 30 μg of genomic DNA from 100 mg of fresh plant tissue per well
- Format: Presto™ 96 Well gDNA Binding Plates
- Elution Volume: 200 ~ 400 µl
- Centrifuge or vacuum manifold protocol
- Storage: dry at room temperature (15-25ºC) for up to 18 months without showing any reduction in performance
Applications
PCR, AFLP/PADP, RFLP, Southern Blotting, Real-time PCR
Components
- GP1 Buffer and GPX1 Buffer
- GP2 Buffer
- GP3 Buffer
- RNase A
- W1 Buffer
- Wash Buffer
- Elution Buffer (10 mM Tris-HCl, pH8.5 at 25°C)
- Presto™ gDNA 96 Well Binding Plates
- 96 Deep Well Plates
- Microtubes (Racked)
- Microtubes (8-strip)
- Caps for Microtubes (8-strip)
Quality Control
M = Geneaid™ 1 Kb DNA Ladder
| Sample | ng/µl | 260/280 | 260/230 | Yield |
| 1. Corn Leaf | 77.7 | 1.80 | 2.02 | 13.6 µg |
| 2. Corn Leaf | 78.8 | 1.80 | 2.05 | 13.8 µg |
| 3. Corn Leaf | 76 | 1.79 | 1.99 | 13.3 µg |
