RedSafe Nucleic Acid Staining Solution
RedSafe™ Nucleic Acid Staining Solution (20,000x) is a new and safe nucleic acid staining solution. It is an alternative to the traditional ethidium bromide(EtBr) stain for detecting nucleic acid in agarose gels. It emits green fluorescence when bound to DNA or RNA. This new stain has two fluorescence excitation maxima when bound to nucleic acid, one centered at 309 nm and another at 419 nm. In addition, it has one visible excitation at 514 nm. The fluorescence emission of RedSafe™ bound to DNA is centered at 537 nm.
RedSafe™ Nucleic Acid Staining Solution (20,000x) is as sensitive as EtBr. The protocol is also similar with a protocol of EtBr. The Ames test proves lower mutation with RedSafe compared with EtBr. In addition, RedSafe™ Nucleic Acid Staining Solution (20,000x) has a negative result in mouse marrow chromophilous erythrocyte micronucleus test and mouse spermary spermatocyte chromosomal aberration test.
RedSafe™ Nucleic acid Staining Solution (20,000x) as compared to EtBr is safer for detecting nucleic acid in agarose gels.
| Product Name: | RedSafe Nucleic Acid Staining Solution | Catalog Number: | 21141 |
| Concentration: | 20,000X | Feature: | Non-toxic, non-mutagenic, non-carcinogenic |
| Excitation Maxima: | 309nm, 419nm | Emission Wavelenght: | 514nm |
| Gel Type: | Nucleic acid gel stain | Target Molecule: | DNA, RNA |
| Ready To Use: | Yes | Detection Method: | Fluorescence |
| Number of Applications: | Sufficient for 200 applications, diluted in 100ml agarose gels | Package: | 1ml x 1vial |
| Storage: | Stable for up to 12 months at room temperature. Stable for up to 24 months at 4℃ | ||
1. DNA
Sensivity of DNA detection of RedSafe Nucleic Acid Staining Solution (20,000x) under UV transmission
Figure1. Gel analysis of serial diluted genomic DNA using RedSafeTM Nucleic Acid Staining Solution (20,000x) and EtBr
2. RNA
Sensivity of RNA detection of RedSafe Nucleic Acid Staining Solution (20,000x) under UV transmission
Figure2. Gel analysis of serial diluted RNA using RedSafeTM Nucleic Acid Staining Solutin (20,000x) and EtBr
